HPLC法测定脉通颗粒中丹酚酸B的含量

Determination of Salvianolic Acid B in Maitong Granules by HPLC

目的建立测定脉通颗粒中丹酚酸B含量的高效液相色谱法。方法采用Diarnonsip@C18（4.6mm×250mm，5μl）；流动相：甲醇-乙腈-甲酸-水（27：10：1：65）；流速：1ml／min；检测波长：286nm。结果丹酚酸B在0．2272-1．1360μg范围内与峰面积具有良好的线性关系，r=0．9999；加样回收率为100．43％，RSD为1．49％（n=5）。结论该法简便、准确，灵敏度高，重复性好，可作为控制脉通颗粒质量的方法。

Aim To establish an RP-HPLC method for quantitative determination of Salvianolic Acid B in Mai tong Granules. Methods The separation was performed on DiamonsiL^TM C18 column （4.6mm×250mm,5μl） with the mobile phase of methanal and Acetonitrile and Formic acid and Water（27： 10：1 ： 65 ） at a flow rate of 1ml/min. The UV detection wavelength was 286nm. Results The linear range of Salvianolic Acid B was 0.2272 - 1.1360μg, r =0.9999. The mean recovery was 100.43% , RSD 1.49% （ n =5）. Conclusion This method is simple,accurate, sensitive and with good reproducibility.