常规放免法测定IAA呈负值的血清中含大量胰岛素抗体

INSULIN AUTOANTIBODIES IN SERA OF INDIVIDUALS WITH NEGATIVE VALUES IN IAA RADIOIMMUNOASSAY

增加非同位素标记胰岛素的量，对６例在Ｖａｒｄｉ法检测中ＩＡＡ呈负值的Ⅰ型糖尿病人亲属的血清标本进行重复检测。结果发现标本中含大量ＩＡＡ。对上述结果进行了分析和探讨，该方法在Ⅰ型糖尿病危险人群调查中，可提高检出率，避免漏诊。

nsulin autoantibidy(IAA) is one of the most important markers for detection of population at risk to develop into Type I diabetes. In screening the subjects and following up their conditions, the authors re-examined some sera with IAA radioimmnoassasy by using different concentrations of unlabeled insulin. The results of these subjects were compared with those of normal controls and IAA positive diabetics. It was confirmed that there was actually a large amount of IAA in the sera of individuals with negative values in standard IAA radioimmunoassay. Only in this group, the levels of IAA varied with increased amount of unlabeled insulin (P<0.01). Should similar events be confronted, it is advisable to increase the amount of unlabeled insulin in IAA radioimmunoassay on purpose to avoid missed diagnosis.