四川部分地区乙型肝炎病毒基因分型分析

Distribution of hepatitis B virus genotypes and its clinical significance in some areas of Sichuan Province

目的了解四川部分地区乙肝病毒(HBV)基因型分布情况。方法选择390例经荧光PCR法定量HBV-DNA含量>104 copies/ml的感染者血清,采用聚合酶链反应-酶联免疫吸附试验(PCR-ELISA)法检测HBV基因型。结果390例患者中B型186例(47.7%),C型142例(36.4%),BC混合型54例(13.8%),A型3例(0.7%),5例测序待定。C型HBV-DNA水平和HBeAg阳性率分别为(7.59±1.18)log值和75.58%,明显高于B型的(5.36±1.10)log值和45.90%(P<0.01);C型ALT、AST、TBIL的水平均高于B型(P<0.05)。结论四川部分地区乙肝病毒基因型以B、C型为主,B型占优势,C型次之。

Objective To investigate tile distribution of hepatitis B virus genotypes in some areas of Sichuan Province and its clinical significance. Methods Serum samples were collected from 390 HBV-DNA 〉 104 eopies/ml with HBV infection in Chengdu, Nanchong, Zigong, Dazhou, Leshan, Yibin, Panzhihua, and Luzhou. HBV genotypes were determined by PCR- ELISA. Results Of the 390 patients, 186（47.7% ） were genotype B, 142（36. 4% ） were genotype C, 54（ 13.8% ） were mix-infected by B＆C, 3 （0. 7% ） were genotype A and 5 samples were not determined by PCR-EL1SA that needed to determine by sequencing. The serum level of HBV-DNA and HBeAg positive rate in patients of genotype C was 7.59±1. 18 and 75.58% , higher than 5.36±1. 10 and 45.90% in those of genotype B（ P 〈0. 01） ,and the levels of ALT, AST, TBIL in genotype C samples were also higher than those of genotype B ones （ P 〈 0. 05 ）. Conclusion Genotype B is a predominated genotype and genotype C is the second in part Sichuan areas. The damage to liver indue.ed by genotype C is more severe than that of genotype B. The reaction and prognosis are relatively better in patients with genotype B than those with genotype C.