沙棘酵母酵母泥中活性多糖的提取及理化性质分析

The Active Polysaccharide Extraction and Its Physics and Chemistry Characteristic Analysis from the Sea-buckthorn Yeast

该文分别采用酸法、碱法、超声波破碎法、Sevage脱蛋白法等多种方法从沙棘酵母酵母泥中提取活性多糖β-1,3-D-葡聚糖,并对所得产品的多糖含量及其组成和理化性质进行分析。试验结果表明,利用酸法可从沙棘酵母提取β-1,3-D-葡聚糖得率高于碱法。通过对多糖的单糖组成分析得知,酸法提取多糖中只含有单一的葡聚糖,而碱法提取所得多糖产品中除含有葡聚糖外还含有甘露聚糖。

By using acid process, alkaline process, ultrasonic wave cracking process, and Sevage albumenose-free method, the active polysaeharide β-1.3-D-dexlran was extracted from the sea-huckthorn yeast, also the polysaecharide content and the composition as well as the physics and chemistry characteristics were analysed, the result indicated that the sea-huekthorn yeast β-1 . 3- D- dextran extraetion rate is higher by using alkaline process than that of acid process. Also. there is only dextran as polysaecharide using acid process extraction. But dextran as well as mannan was got by using alkaline process. This provides the foundation for further using the sea-buckthorn yeast polysaccharide.