CYP1A1和NAT2基因多态性与肾癌易感性关系的病例对照研究

Association of genetic polymorphisms in CYP1A1 and NAT2 with susceptibility to renal cancer:a case control study

目的:探讨致癌物CYP1A1和NAT2基因多态性对肾癌易感性的影响。方法:利用限制性片段长度多态性-聚合酶链反应(RFLP-PCR)方法,检测病例和对照组细胞色素P450酶基因CYP1A1 MspⅠ位点和N-乙酰基转移酶2(NAT2)基因的多态性情况。结果:与对照组相比,病例组CYP1A1(W/M)及NAT2(intermediate)基因型分布差异均存在统计学意义(P<0.05)。携带CYP1A1(W/M)或NAT2(intermediate)基因型的个体患肾癌的危险度升高,比值比(OR)分别达到2.487(1.493～4.142)和1.970(1.128～3.442)。多因素分析结果显示携带CYP1A1(W/M)及NAT2(intermediate)基因型的吸烟个体具有肾癌易感性。结论:CYP1A1(W/M)和NAT2(intermediate)基因型均是肾癌的危险因素,两者存在交互作用,且均与吸烟有协同作用。

Objective： To study the effect of genetic polymorphisms of cytochrome P4501A1 （CYP1A1）, N- aeetyltransferase 2 （NAT2） on the susceptibility to renal cancer. Methods： The genetic polymorphisms of CYPIA1 and NAT2 were examined in the renal cancer patients and controls using restriction fragment length polymorphism-polymerase chain reaction （RFLP-PCR） methods. Results： The distribution of CYP1A1 （W/M） and NAT2 （intermediate） genotypes was significantly different between the renal cancer patients ancl controls （P〈0.05）. Individuals carrying CYP1A1 （W/M） or NAT2 （intermediate） genotypes had an increased risk for renal cancer（OR=2. 487, 95% CI.. 1. 493-4. 142; OR： l. 970, 95% CI.. 1. 128-3. 442,respectively）. Multivariate analysis showed increased risk for renal cancer patients carrying CYP1A1 （W/M） or NAT2 （intermediate） genotypes and those who smoke. Conclusion： The genotypes CYP1A1 （W/M） and NAT2 （intermediate） are the risk factors of renal cancer, and the 2 genotypes have a interactive effect and both have a joint effect with smoking.