控释性可注射牙槽骨修复材料中小分子单体的体外细胞毒性研究

In vitro cytotoxicity of low molecular weight monomers in a novel injectable alveolar replacement material for controlled delivery of tetracycline.

目的:研究控释性可注射牙槽骨修复材料中小分子单体的体外细胞毒性。方法:用细胞培养液分别浸提4种小分子单体NVP、NMP、BPO、DMT 30 min和24h,配置成不同浓度的浸提液(0.1～100mg/ml),将小鼠成纤维细胞(L-929)在浸提液中分别培养24h,通过Alamar Blue比色法,检测4种小分子单体对L-929细胞相对增值率的影响,评价其细胞毒性。结果:种小分子单体的细胞毒性均与时间及浓度有密切关系,浸提时间越长、浸提液浓度越高,其细4胞毒性越强。结论:控释性可注射牙槽骨修复材料中小分子单体的细胞毒性需受到重视,研制时应采取措施提高其生物相容性。

Objective：To investigate the in vitro cytotoxicity of four kinds of low molecular weight monomers in a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Method：Cell culture medium was exposed to the low molecular weight monomers （0.1～100 mg/ml） for two different extraction periods：30 min and 24 h. The L-929 mouse fibroblasts were then exposed to the treated cell culture medium for 24 h. Finally,cell viability and growth were assessed using Alamar Blue assay. Result：Four kinds of low molecular weight monomers exhibited a time-and dose-dependent cytotoxic response. Cell viability increased with further dilutions of the extraction media and for the 30 min extraction period. Conclusion：These results show that there are concerns regarding the biocompatibility of low molecular weight monomers but also sheds light onto potential mechanisms to reduce the cytotoxic effects.