摘要
目的了解子宫内膜异位症(Em)患者的在位内膜在分泌RANTES及对单核细胞的趋化活性是否有别于非Em患者子宫内膜。方法白细胞介素(IL)-1β刺激培养Em与非Em患者子宫内膜基质细胞,分别在刺激培养后12、24、36、48、60、72、84和96 h取上清酶联免疫吸附试验(ELISA)检测RANTES水平;Boyden小室趋化实验分析刺激培养上清液对单核细胞的趋化活性及RANTES趋化单核细胞的能力。结果Em在位内膜从刺激培养60 h始,RANTES分泌量及趋化单核细胞的能力显著高于非Em在位内膜;Em在位内膜基质细胞刺激培养60 h上清所趋化的单核细胞中有55%是由RAN-TES趋化而来。结论在相似的培养条件下,Em患者的在位内膜基质细胞RANTES的表达模式和趋化单核细胞的活性不同于非Em患者子宫内膜。
Objectives: To evaluate if the expression and chemotactic activity of RANTES are different in IL-1β treated autologous eutopic endometrial stromal cells from endometriosis patients compared to non-endometriosis eutopic endometrium. Methods: Eutopic endometrial stromal cells from patients with or without endometriosis were collected and cultured. IL-1β was added to induce the secretion of RANTES. Concentrations of RANTES in the culture medium at 12 h, 24 h, 36 h, 48 h, 60 h, 72 h, 84 h and 96 h were measured with a sandwich ELISA. U937 monocyte ehemotactic activity was assayed in Boyden Chambers. Results: The RANTES secreted by the stromal cells of autologous eutopie endometrium from endometriosis patients was increased significantly than that from non-endometriosis women after 60 h of culture. The RANTES accounts for the majority(55 %) of the monocyte chemotactic activity in autologous eutopie endometrial stromal cells conditioned media. Conclusions: The expression of RANTES and its monocyte chemotactic activity of autologous eutopie endometrium in the endometriosis patients were different significantly from those in non-endometriosis women.
出处
《生殖医学杂志》
CAS
2008年第5期356-360,共5页
Journal of Reproductive Medicine
基金
国家自然科学基金(30600671)
江苏省自然科学基金(BK2006577)资助