子宫内膜异位症患者在位内膜分泌RANTES及对单核细胞的趋化活性

The expression of RANTES and its monocyte chemotactic activity in the stromal cells of autologous eutopic endometrium of the patients with endometriosis

目的了解子宫内膜异位症(Em)患者的在位内膜在分泌RANTES及对单核细胞的趋化活性是否有别于非Em患者子宫内膜。方法白细胞介素(IL)-1β刺激培养Em与非Em患者子宫内膜基质细胞,分别在刺激培养后12、24、36、48、60、72、84和96 h取上清酶联免疫吸附试验(ELISA)检测RANTES水平;Boyden小室趋化实验分析刺激培养上清液对单核细胞的趋化活性及RANTES趋化单核细胞的能力。结果Em在位内膜从刺激培养60 h始,RANTES分泌量及趋化单核细胞的能力显著高于非Em在位内膜;Em在位内膜基质细胞刺激培养60 h上清所趋化的单核细胞中有55%是由RAN-TES趋化而来。结论在相似的培养条件下,Em患者的在位内膜基质细胞RANTES的表达模式和趋化单核细胞的活性不同于非Em患者子宫内膜。

Objectives： To evaluate if the expression and chemotactic activity of RANTES are different in IL-1β treated autologous eutopic endometrial stromal cells from endometriosis patients compared to non-endometriosis eutopic endometrium. Methods： Eutopic endometrial stromal cells from patients with or without endometriosis were collected and cultured. IL-1β was added to induce the secretion of RANTES. Concentrations of RANTES in the culture medium at 12 h, 24 h, 36 h, 48 h, 60 h, 72 h, 84 h and 96 h were measured with a sandwich ELISA. U937 monocyte ehemotactic activity was assayed in Boyden Chambers. Results： The RANTES secreted by the stromal cells of autologous eutopie endometrium from endometriosis patients was increased significantly than that from non-endometriosis women after 60 h of culture. The RANTES accounts for the majority（55 %） of the monocyte chemotactic activity in autologous eutopie endometrial stromal cells conditioned media. Conclusions： The expression of RANTES and its monocyte chemotactic activity of autologous eutopie endometrium in the endometriosis patients were different significantly from those in non-endometriosis women.