乙烯-醋酸乙烯共聚物涂层表面抗体的固定及体外细胞黏附研究

Immobilization of CD34 Antibodies onto Ethylene-vinyl Acetate Copolymer Coatings and in Vitro Cell Attachments

表面快速内皮化是预防支架植入后再狭窄的新途径。本实验分别将仿生海洋生物粘性多肽及CD34抗体固定到支架涂层材料—乙烯-醋酸乙烯共聚物(EVA)涂层表面,并对其体外细胞黏附进行了研究。结果表明:多肽及抗体能够有效地促进内皮细胞的黏附和增殖,提高细胞活性,增加细胞与EVA基体的结合强度。随着多肽浓度的增加,内皮细胞的黏附增加,但浓度过高,反而抑制细胞的黏附;提高抗体浓度,可以增加抗体的固定及细胞的黏附。

Rapid re-endothelialization of implanted coronary artery stent is a new way for preventing restenosis. In this paper, adhesive polypeptide mimics of marine organism and antibodies potentially selective targeting with CD34 antigens on endothelial cells （ECs） were immobilized onto ethylene-vinyl acetate copolymer （EVA） coatings for improving the re-endothelialization process. The results showed that the attachments, growths, viabilities, as well as cell retentions of ECs on EVA were improved after modification. The immobilized polypeptide promoted the attachments of ECs, but overmuch polypeptide would restrict the attachments. With the increase of antibody concentration, the immobilized antibodies and cell attachments were improved.