摘要
目的观察201B中药合剂防治大鼠抗肾小球基底膜(GBM)肾炎的疗效。方法建立大鼠抗GBM肾炎模型。实验分3组:肾炎+201B处理组、肾炎对照组及正常对照组。大鼠一次性尾静脉注射抗GBM抗血清后即刻给予201B中药合剂(0.46g/(kg·d),灌胃,至第21天。对照组则给予等量的生理盐水。定期于第4天、第14天和第21天,检测大鼠尿蛋白、血清肌酐和尿素水平,观察肾组织病理学改变。结果肾炎对照组鼠注射抗血清后于第4天即出现异常蛋白尿,血清肌酐和尿素亦持续上升;肾小球内可见细胞数增加和新月体形成,肾小管内大量蛋白管型,GBM呈不规则增厚。而上述病变经201B中药合剂处理后均明显好转。结论201B中药合剂能够明显改善大鼠抗GBM肾炎的肾功能。
Objective To investigate the effects of 201B on anti-glomerular basement membrane (GBM) nephritis in rats. Methods Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody in jection in SD rats in this study. The rats were divided into three groups: 201B treated group(0.46gokg-lod- 1 ,ig), untreated nephritis control group and normal control group. 201B was administered daily after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected. Results Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4th days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephriticrats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. But, all pathological changes obviously turned for the better in 201B treated group. Conclusion 201B could inhibit the progression of rats anti-GBM nephritis.
出处
《中国实用医药》
2008年第27期17-18,共2页
China Practical Medicine