注射用大川芎中药材的质量研究

The quality study on the herbs used in Rhizoma Chuanxiong injection

目的对注射用大川芎中所用原药材进行了质量研究。建立了川芎药材和天麻药材的TLC鉴别方法,并对样品进行了鉴别。方法建立了川芎药材中阿魏酸及天麻药材中天麻素的HPLC含量测定方法,并对方法学进行了考察。结果在所确立的色谱条件下,阿魏酸在0.0316-0.5056μg范围内,峰面积和进样量线性关系良好,精密度RSD=1.25%,平均回收率为97.80%（n=9,RSD=1.47%）;天麻素在0.502-10.04μg范围内,峰面积和进样量线性关系良好,精密度RSD=1.21%,平均回收率为98.00%（n=9,RSD=1.39%）。结论供试品色谱中,在与对照药材及对照品色谱相应的位置上,显相同颜色的斑点,阴性样品无干扰。

Objective To study the quality on the herbs used in Rhizoma Chuanxiong injection. Methods Rhizoma Chuanxiong and Rhizoma Gastrodiae were identified with TLC methods. The methods of HPLC were built up to determine the content of the two main ingredients in these two herbs. Results Under the identified chromatography condition , the peak area of Ferulic acid is in good linearity to its concentration in the range of 0. 0316 -0. 5056 μg. The precision （RSD） is 1.25% and the average recovery is 97.80% （n = 9 RSD = 1.47% ）. As to Gastrodin ,in the range of 0. 502 - 10. 04ug,the linearity between peak area and concentration is satisfactory. The precision is 1.21% and the average recovery is 98. 00% （n = 9 RSD = 1.39% ）. Conclusion The two methods above were accurate, reliable and repeatable.