抑制趋化因子受体CCR5减轻小鼠同种异体移植心脏急性排斥反应的研究

Acute cardiac aliograft rejection in mice is aUeviatedby inhibition of CCR5

目的研究抑制趋化因子受体CCR5减轻小鼠同种异体移植心脏急性排斥反应的作用机制。方法采用小鼠颈部心脏移植模型，将96只小鼠用随机数字表法分为4组，每组24只，供、受者各12只，A组术后给予anti-CCR5mAb和CsA，B组术后给予anti—CCR5 mAb，C组术后给予CsA，D组为对照组，术后给予生理盐水。于术后第7d取各组移植心组织6例，检测CCR5及IL-2和IL-10的表达差异，其余6例用于观察移植心脏存活时间。结果A、B、C组小鼠移植心脏存活时间明显延长，其CCR5及IL-2的表达较D组明显减少，IL-10的表达明显增加。结论抑制趋化因子受体CCR5对同种异体移植心脏有明显的保护作用，可能与细胞因子的表达有关。

Objective To investigate the machnism of anti-CCR5 mAb alleviating acute cardiac allograft rejection in mice. Methods We study intragraft event in four groups. Group A are cardiac allograft recipients with anti-CCR5 mAb and CsA,group B are cardiac allograft recipients with anti-CCR5 only,group C are cardiac aUograft recipients with CsA only,group D are control group, cardiac allograft recipients with 0.9% sodium chloride injection. We study the mechanism on day 7 posttransplant. Results Mice treated with a neutralizing mAb to CCR5 show enhanced aUograft survival, decreased level of CCP,5 and IL-2 and increased level of IL- 10. The beneficial effects of targeting of CCR5 are markedly synergistic with the effects of cyclosporine, resulting in alleviated acute cardiac allograft rejection. Conclusion We conclude that the inhibition of CCR5 have a markedly protection to cardiac allograft, this may due to the change in expression of some cytokine.