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Na^＋／H^＋逆向转运蛋白NhaH短的C末端亲水域介导盐抗性和pH感应 被引量：1

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摘要 Na+/H+逆向转运蛋白在维持细胞质低的Na+浓度和pH稳态等生命活动过程中扮演重要角色,然而,到目前为止,对原核微生物Na+/H+逆向转运蛋白的C末端亲水域的结构和功能还知之甚少.我们曾从达坂喜盐芽孢杆菌(Halobacillus dabanensis)D-8菌株中克隆得到第一个中度嗜盐菌来源的Na+/H+逆向转运蛋白基因nhaH.疏水性分析表明,NhaH的C末端亲水域只包含9个氨基酸残基(395PLIKKLGMI403),大大短于与之高度同源的SynNhaP1和ApNhaP的C末端亲水域.本研究采用PCR方法,构建NhaH蛋白的C末端亲水域缺失突变体nhaH-C.耐盐性实验表明,C末端亲水域的缺失显著地抑制大肠杆菌缺陷株KNabc的互补能力.基于荧光强度的翻转膜活性测定结果显示,NhaH?C的Na+/H+和Li+/H+逆向转运蛋白活性显著低于NhaH,而且前者的Na+/H+逆向转运蛋白活性向酸端偏移,表明Na+/H+逆向转运蛋白NhaH短的C末端亲水域在阳离子结合、转运和pH感应上起重要作用.
作者 杨礼富 张博 王磊 杨苏声
机构地区 中国农业大学生物学院微生物与免疫学系
出处 《科学通报》 CAS CSCD 北大核心 2008年第19期2313-2317,共5页 Chinese Science Bulletin
基金 国家高技术研究发展计划(批准号:2003AA241150) 国际科技合作项目(批准号:2006DFA31060)资助
关键词 达坂喜盐芽孢杆菌 NA^+/H^+逆向转运蛋白 C末端亲水域 盐抗性 pH感应
分类号 Q51 [生物学—生物化学]
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参考文献14

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同被引文献25

  • 1李金耀,张富春,马纪,单文娟,王宾.大肠杆菌Na^+/H^+反向运输体A基因的克隆及序列分析[J].微生物学通报,2004,31(4):34-37. 被引量：2
  • 2INABA M,SAKAMOTO A,MURATA N.Functional expression in Escherichia coli of low-affinity and high-affinity Na+(Li+)/H+ antiporters of Synechocystis[J].Journal of Bacteriology,2001,183:1376-1384.
  • 3YANG L F,JIANG J Q,ZHAO B S,et al.A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8T:cloning and molecular characterization[J].FEMS Microbiology Letters,2006,255:89-95.
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  • 6MOON M S,LEE D H,KIM C K.Identification of bphc gene for metacleavage of aromatic pollutants.from a metagenomic library derived from lake waters[J].Biotechnology Bioprocess Engineering,2004,9(5):393-399.
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  • 9MAJERNIK A,GOTTSCHALK G,DANIEL R.Screening of environmental DNA libraries for the presence of genes conferring Na+(Li +)/H+ antiporter activity on Escherichia coli:characterization of the recovered genes and the corresponding gene products[J].Journal of Bacteriology,2001,183:6645-6653.
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科学通报
2008年 第19期

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