血管内皮生长因子与非霍奇金淋巴瘤浸润的相关性研究

Correlation of vascular endothelial growth factor with metastasis of non-Hodgkin lymphoma

目的检测非霍奇金淋巴瘤（NHL）患者血清中血管内皮细胞生长因子（sVEGF）的浓度，探讨其与NHL浸润的关系。方法采用夹心酶联免疫吸附法（ELISA）检测81例NHL初治患者和36例健康对照sVEGF的水平。同时检测81例NHL初治患者血浆乳酸脱氢酶（LDH）浓度。比较NHL患者与对照组sVEGF的差别及不同临床分期、治疗效果、血浆LDH浓度、sVEGF水平的差别，分析NHL患者sVEGF水平、LDH浓度的相关性。结果NHL患者组sVEGF水平（282．90±29．80）Pg／ml，较对照组升高[（180．20±14．91）Pg／ml]，且差异具有统计学意义（P〈0．05）；Ⅲ、IV期患者sVEGF水平为（304．35±143．21）Pg／ml高于I、Ⅱ期的（257．86±168．10）Pg／ml，但差异无统计学意义；未完全缓解患者组sVEGF水平为（323．26±159．89）Pg／ml，明显高于达完全缓解者组[（228．21±143．21）pg／ml]（P〈0．05）；LDH升高组sVEGF水平（385．40±157．87）pg／ml较LDH正常组（234．18±158．87）Pg／ml明显升高（P〈0．05）；VEGF水平与LDH浓度间有显著的相关性（P〈0．01）。结论血管生成在NHL的侵袭转移机制中具有重要作用；sVEGF水平可作为NHL了解病情、观察疗效和预后的指标。

Objective To investigate the relationship of vascular endothelial growth factor （VEGF） with the invasion and metastasis of non-Hodgkin lymphoma. Methods The levels of serum VEGF was measured by enzyme linked-immunoadsorbent assay （ELISA）. Comparing the parameters between NHL group and the control group. Compared the parameters with different groups divided by clinical stage, the effect of the treatment , the level of plasma LDH and effect of treatment. Analyzed the correlation of the levels of serum VEGF and plasma LDH in patients of NHL. Results The levels of serum VEGF are remarkablely higher in patients than that in the control group （P 〈0.05）. The levels of serum VEGF in the group of Ⅲ, Ⅳ clinical stage are higher than that in the group of I, Ⅱ clinical stage, but no significant difference（P 〉0.05） were observed. The level of serum VEGF is higher in the group of none CR than the group of CR （P 〈0.05）. The level of serum VEGF is higher in the group of non-normal level of plasma LDH than group of normal LDH level（P 〈0.05）. The levels of VEGF and LDH are significantly correlated each other（P 〈0.01）. Conclusion The angiogenesis are important for tumor metastasis in patients of NHL. The serum VEGF can be used as prognosis parameters in patients with NHL.