RP-HPLC法测定猪血浆中头孢喹肟浓度

RP-HPLC determination of cefquinome in pig plasma

目的:建立测定头孢喹肟血药浓度的 RP-HPLC 法。方法:血浆样品经乙腈沉淀蛋白后取上清进样测定,色谱柱为 C_(18)柱(150 mm ×4.6 nm,5 μm),流动相为磷酸盐缓冲液(0.83%磷酸用三乙醇胺调 pH 为2.8)-乙腈(86:14),流速为1 mL·min^(-1),检测波长270 nm,柱温25℃。结果:头孢喹肟血药浓度在0.05～20 μg·mL^(-1)范围内线性关系良好(r=0.9994);最低检测限为0.05μg·mL^(-1);方法回收率88.0%～96.8%;日内 RSD 和日间 RSD 均小于10%。结论:方法快速简便、灵敏准确,适用于头孢喹肟在动物体内的药物动力学研究。

Objective：To establish an HPLC method for the determination of cefquinome in pig plasma. Method： Plasma sample was deproteinated by acetonitrile. The chromatographic separation was performed on a C18 column （ 150 mm ×4. 6 mm,5μm）. A mixture of phosphate buffer （0. 83% phosphoric acid,adjusted to pH 2.8 by trieth- anolamine） and acetonitrile （86： 14） , as mobile phase, was delivered at a flow rate of 1 mL· min-l. The detection wavelength was 270 nm. The column temperature was 25 ℃. Results：Excellent linear relationship was obtained in the range of 0.05 μg · mL^ - 1 to 20 μg · mL^-1 （ r = 0. 9994 ）. The detection limit was 0. 05 μg · mL^ - 1. Method recovery was 88.0% -96.8%. The intra- day and inter- day RSD was less than 10%. Conclusion：The method is rapid, simple, sensitive and accurate, it can be used for study on pharmacokinetics of cefquinome in animals.