金银花药材的HPLC特征图谱研究

Study on HPLC-specific chromatograms of Flos Lonicerae Japonicae

目的:建立金银花药材的HPLC特征图谱,为金银花药材的质量鉴定提供依据。方法:采用Phnomenex Gemini C_(18)色谱柱(250 mm×4.6 mm,5μm),以0.5%磷酸-乙腈为流动相梯度洗脱,流速为1 mL·min^(-1),柱温40℃。检测器为紫外检测器和电化学检测器。结果:得到了分离度较好的HPLC-UV(238 nm)和HPLC-ECD(250 mV)2种特征图谱,两者具有良好的相关性和互补性。分别识别了17个和9个特征共有峰。结论:该方法稳定、准确、可靠,可用于金银花药材的质量控制。

Objective：To establish the HPLC specific chromatograms of Flos Lonicerae Japonicae, it may provide the basis for identification for quality of Flos Lonicerae Japonicae. Methods： Chromatographic analysis was achieved on a Phnomenex Gemini C18 column（250 mm ×4. 6 mm,5 μm） using a mobile phase of 0. 5% phosphoric acidacetonitrile eluted with a linear gradient. The detectors were consisted of UV and ECD detector. The flow rate was maintained at 1.0 mL· min^-1, with the column temperature at 40℃. Results：The method provided two kinds of specific chromatogram with excellent separation, which were HPLC - UV （ 238 nm） and HPLC - ECD （ 250 mV） specific chromatograms. They had a good correlation and complementation with each other. Seventeen common peaks and nine common peaks were confirmed in each specific chromatogram, respectively. Conclusion：The method is stable, accurate and creditable. It can be used for the quality control of Flos Lonicerae Japonicae.