反相HPLC法同时测定大柴胡汤中3种黄酮苷的含量

RP-HPLC simultaneous determination of three flavonoid glycosides in Dachaihu decoction

目的:建立反相HPLC法同时测定大柴胡汤中柚皮苷、橙皮苷、黄芩苷的含量。方法:采用ZORBAX Eclipse XDB-C_(18)反相色谱柱(150 mn×4.6 mm,5 μm),以乙腈-1.5%醋酸溶液(20:80)为流动相,流速0.8 mL·min^(-1),检测波长280 nm,柱温为室温。结果:在18 min内柚皮苷、橙皮苷、黄芩苷达到良好分离,并在4.0～254.0 μg·mL^(-1)(r=0.9999),2.3～141.0μg·mL^(-1)(r=0.9989),1.3～172.0μg·mL^(-1)(r=0.9999)浓度范围内呈良好线性关系;加样回收率(n=6)分别为99.2%,99.2%,99.7%。结论:本方法简便、快速,适合于大柴胡汤的质量控制。

Objective ：To develop an RP - HPLC method for simultaneous determination of naringin, hesperidin and baicalin in Dachaihu decoction. Method：A ZORBAX Eclipse XDB -C18 （150 mm × 4. 6 mm,5 μm） column was used,and the solvent system composed of CH3CN- 1.5% HAe solution （20：80, v/v） was used as the mobile phase. The flow rate of the mobile phase and the detection wavelength were set at 0. 8 mL·min ^- 1 and 280 nm, respectively. Results：Naringin, hesperidin and baicalin were separated excellently in less than 18 min with the linear rage of 4. 0 -254. 0 μg ·mL^-1 （r =0. 9999） ,2.3 - 141.0 μg ·mL^-1 （r =0. 9989） and 1.3 - 172. 0 μg · mL^-1 （r = 0. 9999）, with the recoveries （ n = 6 ） of 99.2% ,99.2% and 99.7 % , respectively. Conclusions ： The established method is fast, simple, and may be suitable for quality control of Dachaihu decoction.