毛细管电泳在线富集技术及其在生物样品中药物分析的研究进展

The advance and application of on-line preconcentration technology in capillary electrophoresis for pharmaceutical analysis in biological sample

毛细管电泳在线富集技术具有灵敏度高、操作简单、成本低廉、试剂消耗少等特点,主要包括等速电泳、电堆积富集、扫集法、pH调制堆积和在柱色谱预浓缩等,这些方法可以提高检测灵敏度,降低样品浓度检测限,拓展毛细管电泳在众多领域的应用。本文综述了近年来毛细管电泳在线富集技术在生物样品中痕量药物分析的应用及研究进展。

The method of sample on - line preconcentration technology in capillary electrophoresis is characteristic with high sensitivity, simple operation and low cost. The technology is mainly including isotachophoresis, sample stacking, sweeping, pH - mediated stacking and on - line chromatography preconcentration. These methods can improve sensitivity,degrade the limit of quantitation and expand application extent of capillary electrophoresis. In this paper, the application and advance of on - line preconcentration technology in capillary electrophoresis for pharmaceutical analysis in biological sample are reviewed.