乳腺癌组织中Fas和CTLA-4及RhoBTB2基因表达及其与临床病理因素的关系

Expressions of Fas, CTLA-4 and RhoBTB2 genes in breast carcinoma and their relationship with clinicopathological factors

目的探讨乳腺癌组织中Fas、CTLA-4和RhoBTB2基因表达的临床意义。方法应用半定量逆转录聚合酶链反应（RT—PCR），检测60例原发乳腺癌患者癌组织和30例乳腺正常腺体组织中Fas、CTLA-4和RhoBTB2基因的mRNA表达，分析其与患者临床病理因素的关系。结果乳腺癌组织中Fas、CTLA-4及RhoBTB2基因的相对表达量分别为0．699±0．285、1．045±0．302和0．625±0．160，而在乳腺正常腺体组织中分别为0．502±0．178、0．418±0．140和0．843±0．218，差异有统计学意义（P〈0．01）。腋淋巴结转移患者乳腺癌组织中Fas基因的表达（0．782±0．313）显著高于无转移患者（0．557±0．146，P〈0．01），Ⅱ期患者乳腺癌组织中CTLA-4基因的表达（0．978±0．330）低于Ⅲ期患者（1．134±0．240，P〈0．05），浸润性导管癌患者乳腺癌组织RhoBTB2基因的表达（0．597±0．157）低于浸润性小叶癌（0．717±0．145，P〈0．05）。Fas、CTLA-4和RhoBTB2的mRNA表达均与乳腺癌患者年龄、雌激素受体（ER）、孕激素受体（PR）、人表皮牛长因子受体2（Her-2）状态和生存时间无关，且3者间亦尤显著相关（P〉0．05）。结论Fas、CTLA-4和RhoBTB2的mRNA表达均与乳腺癌发生、发展有关。Fas mRNA表达与腋淋巴结转移有关，CTLA-4 mRNA表达与乳腺癌分期有关，RhoBTB2 mRNA表达与乳腺癌病理类型有关。

Objective The purpose of this study was to explore the clinical significance of expression of Fas, CTLA-4 and RhoBTB2 genes in breast carcinoma. Methods Semi-quantitative reverse transcriptive polymerase chain reaction （RT-PCR） was used to analyze the mRNA expression levels of the three genes in tumor tissues from 60 patients with primary breast cancer and normal breast tissues of 30 cases. The relationship between gene expression and clinieopathological factors were analyzed and determined. Results The relative expression levels （gray scale ratio between target gene and internal reference gene） of Fas, CTLA-4 and RhoBTB2 genes in breast carcinoma tissues were 0. 699 ± 0. 285, 1.045±0. 302 and 0. 625±0. 160, respectively. In the normal breast tissues, they were 0. 502±0. 178, 0.418±0. 140 and 0. 843 ± 0. 218, respectively. There were statistically significant differences of the expression of those three genes between carcinoma tissues and normal breast tissues （ P 〈 0. 01 ）. The expression level of Fas in carcinoma tissues was significantly higher in lymph node matastasis positive patients （0. 782 ± 0. 313） than that in node-negative patients （0. 557 ± 0. 146, P 〈 0. 01 ）. The expression level of CTLA-4 gene in carcinoma tissues was lower in Ⅱ stage patients （0.978 ±0. 330） than that in Ⅲ stage patients （1. 134 ± 0. 240, P 〈 0.05 ）. The expression level of RhoBTB2 gene was lower in invasive ductal carcinoma （0. 597 ± 0. 157 ） than that in invasive lobular carcinoma （0. 717 ± 0. 145, P 〈 0.05 ）. There were no correlations of expression of the three genes at mRNA level and age, ER, PR, HER2 status and survival time. Furthermore, no correlation was seen among the three genes expression （ P 〉 0.05）. Conclusion The expression of all the three genes at mRNA level is involved in genesis and progression of breast cancer. There exist correlations between Fas expression and axillary lymph node matastasis, CTLA-4 expression and disease stage, and RhoBTB2 expression and pathological type of breast cancer.