酶解大豆蛋白制取超氧阴离子清除活性肽的研究

Production of superoxide anions scavenging peptide from soybean protein hydrolysates

研究了碱性蛋白酶水解大豆蛋白制备抗氧化活性肽的工艺条件,探讨了其抗氧化活性与蛋白水解度(DH)、TCA-NSI之间的关系。以超氧阴离子清除率为指标,在单因素实验的基础上通过正交实验优化的酶解条件为:反应温度50℃,时间1h,加酶量1.0%,pH9.0,其酶解物超氧阴离子清除率为33.93%,清除活性的IC50为8.014mg/mL,此时DH为18.27%,TCA-NSI为71.81%,PCL为5.47。不同组别实验结果的统计表明,DH、TCA-NSI与酶解物抗氧化活性之间没有直接关联性。

The enzymolysis conditions of producing antioxidation bioactive peptides from soybean protein were studied. The correlation between antioxidation and DH, TCA-NSI of hydrolysates was discussed. The results showed that the optimum conditions for enzymatic hydrolysis were： temperature 50℃, E/S 1.0%, pH 9.0 and reaction time 1h, in which the superoxide anions scavenging rate of the hydrolysates was 33.93%, IC50 was 8.014mg/mL, DH was 18.27%, TCA-NSI was 71.81% and PCL was 5.47. The analysis showed that correlation between antioxidation of hydrolysates and the DH, TCA-NSI was not distinctive.