摘要
目的观察高糖(培养)对离体雪旺细胞活性和增殖能力的影响,探讨周围神经再生时糖尿病性损伤的可能机制。方法采用brockes改良法从新生Wistar乳鼠坐骨神经组织中分离纯化雪旺细胞。实验分为三组:采用正糖(含5mmol/L葡萄糖)为对照组,高糖(含30mmol/L葡萄糖)为高糖组,高糖高渗(含30mmol/L葡萄糖+100mmol NaCl)为高渗组;每组6对。采用XTT法测定雪旺细胞活性,^3H-TdR掺入法检测雪旺细胞的增殖能力。结果高糖组、高渗组同对照组比较,细胞活力明显减少,差异有统计学意义(P〈0.05);高糖组与对照组比较,细胞增殖能力受到抑制,两组差异有统计学意义(P〈0.01);高渗组与高糖组比较,细胞增殖能力明显受到抑制,差异有明显统计学意义(P〈0.01)。表明高糖对离体雪旺细胞活性和增殖能力均具有显著抑制作用,但较高渗同时存在时为轻。结论周围神经再生时糖尿病性损伤可能与高糖状态影响雪旺细胞的活力和增殖能力有关。
Objective To examine the effect of culture with high glucose on activity and proliferation of Schwann cells and to investigate the possible mechanism of diabetic lesion in peripheral nerve regeneration. Methods Schwann cells were isolated from sciatic nerves of newborn Wistar suckling rats and purified using modified Brockes method. The cells were then cultured under various conditions according to group assignment: control group (normo-glueose, 5 mmol/L glucose), hyper-glucose group (30 mmol/L glucose), and hyper-osmotic group(30 mmol/L glucose+100 mmol NaCI). XTT method and 3H-TdR incorporative method were adopted to measure the activity and proliferation ability of Schwann cells. Results As compared with the control group, the cellular activity in the hyper-glucose and hyper-osmetic group was markedly attenuated (P〈0.05). Proliferation ability of the Schwarm cells in the hyper-glucose group was markedly inhibited (P 〈 0. 01 ) as compare to those in the control group. Proliferation ability of the Schwann cells in the hyper-osmotic group was even lower than that of the hyper-glucose group, indicating that high glucose had a marked inhibitory effect on the activity and proliferation ability of Schwann cells. The presence of hyperosmotic state further attenuated cell activity and proliferation. Conclusion Diabetic lesion in peripheral nerve regeneration is likely related to the inhibitory effect of high glucose on the activity and proliferation ability of Schwarm cells.
出处
《中华手外科杂志》
CSCD
北大核心
2008年第5期300-302,共3页
Chinese Journal of Hand Surgery
基金
上海市科学技术委员会重点基金资助项目(014319321)
关键词
周围神经
再生
许旺细胞
高糖
Peripheral nerves
Regeneration
Schwann cells
High glucose