十二烷基硫酸钠抗牙菌斑作用的实验研究

Effects of sodium doecyl sulfate on the artificial dental plaque in chemostat

十二烷基硫酸钠（ｓｏｄｉｕｍｄｏｅｃｙｌｓｕｌｆａｔｅ，ＳＤＳ）常作为去垢剂广泛地应用于牙膏之中。已证实ＳＤＳ与羟基磷灰石（ＨＡ）有高度亲合力，通过ＳＤＳ与ＨＡ的结合，干扰蛋白质对ＨＡ的吸附，从而影响牙面获得性膜的形成。为进一步了解ＳＤＳ的抑菌和抗菌斑作用，本研究利用口腔恒化器和扫描电镜及同位素标记液闪检测技术，对ＳＤＳ的抗菌斑作用进行了观察。结果显示，经ＳＤＳ处理后的牙釉质表面没有成熟的牙菌斑形成，仅有少数细菌散在附着，其放射性明显少于对照组。提示ＳＤＳ具有抗菌斑作用，是牙膏中的天然抗菌斑成分之一。

Sodium doecyl sulfate (SDS) is widely used as a detergent in dentifrices. It has been shown to interfere with the protein adsorption to hydroxyapatite (HA), and inhibit acquired pellicle formation. The aim of the present study was to examine the effects of SDS on the artificial dental plaque in chemostat. The amount of the3Hlabelled bacteria adhered on the enamel fragment surface was determined with scintillometer. The artificial dental plaque was observed under the scanning electron microscope. The results showed that enamel fragments treated with SDS adsorbed less bacteria than untreated ones, and had no plague formed. It suggested that SDS can inhibit the bacterial adherence on enamel surface and thus reduce dental plaque formation.