龈沟液中Ⅰ型胶原自身抗体的检测

Antibody to Collagen Type Ⅰ in Periodontitis

本研究用间接ELISA法检测了成人牙周炎（AP）27例44牙、快速进展注牙周炎（RPP）10例26牙及牙周健康对照（H）12例18牙龈沟液中的Ⅰ型胶原自身抗体（IgG）。结果AP、RPP及H各组的Ⅰ型胶原抗体检出率分别27．3％，26.9％和22.2％。各组间的检出率无统计学差异（P＞0．05），且Ⅰ型胶原抗体水平与临床参数也无显著相关。本研究结果不支持牙周炎时机体对Ⅰ型胶原产生了自身体液免疫反应；牙周炎时Ⅰ型胶原的大量破坏与Ⅰ型胶原自身抗体的产生可能无明显关系。

The 88 gingival crevicular fluid (GCF) samples were collected from 27 adult periodonti-tis patients (AP) 44 teeth, 10 rapidly progressive periodontitis patients(RPP)26 teeth and 12 healthyperiodontal controls (H) 18 teeth respectively. IgG to human collagen type Ⅰ were measured in GCFusing an indirect enzyme-linked immunosorbent assay (ELISA). The positive rate of IgG antibody tocollagen in AP、RPP and H groups were 27. 3% 、26. 9%and 22. 2% respectively. The positive1y rateswere not significant (p>0. 05) difference among AP、RPP and H groups. There was also not relation-ship between the levels of IgG to collagen type Ⅰ and the clinic Paraments. The results did not supportthat there was a humoral autoimmunity to type Ⅰ collagen in periodontitis.