毛细管柱气相色谱法测定银杏叶提取物中银杏萜内酯含量

GC Determination of Terpene Lactones in Gingko Leaf Extracts with Capillary Chromatographic Column

对银杏叶提取物中银杏萜内酯(包括BB和GA,GB,GC,GJ)的毛细管柱气相色谱分离条件作了试验,达到银杏萜内酯成分的良好分离。选择了内标物角鲨烷,理论计算并直接测定了银杏萜内酯的相对质量校正因子。采用内标法测定了各组分的含量。BB、GA、GB、GC回收率依次为95.2%,95.5%,94.6%,96.1%。

Chromatographic conditions for separation of terpenes （named in abbreviation as GA, GB, GC, GJ and BB） from extracts of gingko leaves with capillary column were studied, and the terpenes were satisfactorily separated. Squalane was used as internal standard in the determination. The relative mass correction factors were calculated theoratically and also determined experimentally. Tests for recovery of BB, GA, GB and GC were made by addition of standards to a sample of known terpene contents and analyzed by the proposed method, values of recovery obtained were 95. 2%, 95. 5%, 94.6% and 96. 1% respectively.