摘要
研究蒿甲醚(Art)对血吸虫摄入葡萄糖和糖原含量的影响.方法:用Art300mg·kg-1ig治疗感染小鼠后24-48h取虫,作体外培养,测定♀、♂虫糖原含量,对[U14C]葡萄糖的摄入及[U14C]葡萄糖掺入虫糖原的量.结果:感染小鼠用Art治疗后24-48h取虫作体外培养1-24h,♂和♀虫的糖原含量分别减少27%-61%和39%-78%;6组♂虫中,仅3组的葡萄糖摄入减少23%-35%,而♀虫各组的均明显减少,减少率为18%-38%.除2组♂虫外,[U14C]葡萄糖掺入虫的糖原量无明显变化.结论:Art引起血吸虫糖原的减少,可能与干扰糖酵解而不是与葡萄糖的摄入有关.
IM: To study the effect of artemether (Art) on glucose uptake and glycogen content in schistosomes. METHODS: Schistosomes recovered from mice treated intragastrically with Art 300 mg ·kg -1 for 24-48 h, were incubated in the drug free medium containing [U 14 C]glucose 11 1 MBq ·L -1 . The glycogen content, [U 14 C]glucose uptake, and incorporation of [U 14 C]glucose into worm glycogen in both ♂ and ♀ worms were determined. RESULTS: When above mentioned schistosomes were exposed to drug free medium containing [U 14 C]glucose for 1-24 h, the glyco ̄gen con ̄tents of ♂ and ♀ worms decreased 27 %-61 % and 39 %-78 %, respectively. Only 3 out of 6 ♂ worm groups showed 23 %-35 % decrease in glucose uptake, while much less glucose uptake was found in ♀ worms in all groups with reduction rates of 18 %-38 %. Apart from 2 ♂ groups no apparent change in the incorporation of [U 14 C]glucose into the worm glycogen was seen. CONCLUSION: Art induced glycogen reduction in schistosomes was related to an inhibition of glycolysis rather than an interference with glucose uptake.
出处
《中国药理学报》
CSCD
1997年第4期366-367,共2页
Acta Pharmacologica Sinica
关键词
日本血吸虫
葡萄糖
糖原
蒿甲醚
Schistosoma japonicum
glucose
glycogen
carbon radioisotopes
artemether