Effect of artemether on glucose uptake and glycogen content in Schistosoma japonicum

研究蒿甲醚（Ａｒｔ）对血吸虫摄入葡萄糖和糖原含量的影响．方法：用Ａｒｔ３００ｍｇ·ｋｇ－１ｉｇ治疗感染小鼠后２４－４８ｈ取虫，作体外培养，测定♀、♂虫糖原含量，对［Ｕ１４Ｃ］葡萄糖的摄入及［Ｕ１４Ｃ］葡萄糖掺入虫糖原的量．结果：感染小鼠用Ａｒｔ治疗后２４－４８ｈ取虫作体外培养１－２４ｈ，♂和♀虫的糖原含量分别减少２７％－６１％和３９％－７８％；６组♂虫中，仅３组的葡萄糖摄入减少２３％－３５％，而♀虫各组的均明显减少，减少率为１８％－３８％．除２组♂虫外，［Ｕ１４Ｃ］葡萄糖掺入虫的糖原量无明显变化．结论：Ａｒｔ引起血吸虫糖原的减少，可能与干扰糖酵解而不是与葡萄糖的摄入有关．

IM: To study the effect of artemether (Art) on glucose uptake and glycogen content in schistosomes. METHODS: Schistosomes recovered from mice treated intragastrically with Art 300 mg ·kg -1 for 24-48 h, were incubated in the drug free medium containing [U 14 C]glucose 11 1 MBq ·L -1 . The glycogen content, [U 14 C]glucose uptake, and incorporation of [U 14 C]glucose into worm glycogen in both ♂ and ♀ worms were determined. RESULTS: When above mentioned schistosomes were exposed to drug free medium containing [U 14 C]glucose for 1-24 h, the glyco￣gen con￣tents of ♂ and ♀ worms decreased 27 %-61 % and 39 %-78 %, respectively. Only 3 out of 6 ♂ worm groups showed 23 %-35 % decrease in glucose uptake, while much less glucose uptake was found in ♀ worms in all groups with reduction rates of 18 %-38 %. Apart from 2 ♂ groups no apparent change in the incorporation of [U 14 C]glucose into the worm glycogen was seen. CONCLUSION: Art induced glycogen reduction in schistosomes was related to an inhibition of glycolysis rather than an interference with glucose uptake.