乙型肝炎病毒C基因启动子变异、HBV DNA及细胞因子与原发性肝癌的关系

Relationship between HBV c gene promoter mutation and serum HBV DNA contents as well as cytokines with hepatocellular carcinoma

目的:探讨乙型肝炎病毒C基因启动子(HBV BCP)变异、HBV DNA及细胞因子与原发性肝癌(HCC)的关系。方法:将176例HBV慢性感染者分为慢性肝病组(156例)和HCC组(20例)。采用PCR微板核酸杂交结合ELISA检测显示技术检测HBV BCP区核苷酸(nt)1762碱基A→T和1764碱基G→A联合突变。并检测两组患者的血清HBV DNA含量及细胞因子白细胞介素-10(IL-10)、IL-12、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)水平。结果:HBV BCP变异在HCC组的阳性率(70.0%)显著高于慢性肝病组(37.8%,P<0.01)。HCC组的血清HBV DNA含量、TNF-α水平均显著高于慢性肝病组(P<0.05,P<0.01),而血清IL-10、IL-12和IFN-γ水平与慢性肝病组无显著性差异(均P>0.05)。结论:HBV BCP变异与原发性肝癌关系密切,且血清TNF-α及HBV DNA复制水平在原发性肝癌的发生中可能也起到主要的作用。

Objective：To investigate the relationship between hepatitis B virus （HBV）basic core promoter（BCP） mutation and serum HBV DNA contents as well as cytokines with hepatocellular carcinoma（HCC）.Methods：A total of 176 patients with chronic hepatitis B virus infection were divided into two groups：chronic liver diseases group （n=156）and HCC group （n=20）.The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined with the method of polymerase chain reaction （PCR）micruplate hybridization ELISA in all these patients.Serum HBV DNA and interleukin-10 （IL-10）,IL-12,tumor necrosis factor-α （TNF-α） and γ-interferon （IFN-γ）of these patients were detected too.Results：The positive rate of HBV BCP mutation in HCC group was significantly higher than that of chronic liver diseases group （P〈0.01）. The quantity of HBV DNA and the serum level of TNF-α in HCC group were significantly higher than that of chronic liver disease group （P〈0.05,P〈0.01）.The serum levels of IL-10 and IL-12 as well as IFN-γ had no significant difference between these two groups （all P〉0.05）.Conclusion：HCC correlated with HBV BCP mutants.The serum levels of TNF-α and the replication of HBV DNA might have an leading effect on the development of HCC.