荧光法研究硒酶模型化合物消除·OH自由基的作用

Studies on the Scavening Action to Hydroxyl Free Radical of Benzisoselenazolones and Diselenides by Fluorometry

利用Ｆｅｎｔｏｎ反应产生的羟基自由基与苯甲酸生成具有荧光的羟化苯甲酸，用荧光法间接测定羟基自由基的含量．用荧光法研究新合成的５种硒酶模型化合物对羟基自由基的清除作用．结果表明，新合成的硒酶模型化合物对羟基自由基有良好的清除作用．该法具有简便、快速、灵敏等特点．

The scavening action to hydroxyl free radical of benzisoselenazolones and diselenides has been studied by fluorometry. The hydroxyl free radicals were produced via Fenton reaction. The method used provides a sensitive, simple and accurate measurement of the scavening action to hydroxyl free radical for synthetic drugs of highly biological activity. 