气相色谱法检测人尿中R,S-美芬妥英浓度

Determination of Rand SMephenytoin in Human Urine by Gas Chromatography

建立了人尿中Ｒ，Ｓ－美芬妥英的气相色谱（ＧＣ）手性分离与检测方法。在选定的色谱条件下能很好地分离Ｒ，Ｓ－美芬妥英，尿中其它物质无干扰。用外标法定量，线性范围为１２．５～２５００μｇ／Ｌ尿，最小检出浓度为６μｇ／Ｌ尿。方法具有样本制备简便、分析时间短、线性范围宽、干扰少、灵敏和准确等优点，已广泛用于人体内美芬妥英代谢的研究和肝药酶ＣＹＰ２Ｃ１９酶活性的检测。

The levels of R and Smephenytoin in human urine were determined by chiral capillary gas chromatography with nitrogenphosphorus detector. The conditions of the chromatography and detection included a chiral capillary column (ChirasilVal, 25m×025mm id, Alltech), column temperature (T) 190℃, injector T 220℃,detector T 240℃, and the flowrates of 0.85, 3.5 and 120 mL/min for nitrogen, hydrogen and air respectively. Based on the above conditions, the satisfactory separation of R and Smephenytoin was gained, and other interference from urine sample was not found. The linear curves for both tested compounds ranged between 12.5 and 2 500 μg/L, with a minimum detectable concentration of about 6 μg/L. Because of its simplicity, rapidity, sensitivity and accuracy, this method has been extensively used for testing metabolic ability of Smephenytoin polymorphic oxidation in the Chinese populations and for the determination of the activity of hepatic drugmetabolizing enzyme CYP2C19 using mephenytoin S/R ratio of the urine samples of subjects as an indicator.