用固相提取和高效液相色谱法分离检测生物体液中7种氨基甲酸酯类农药

Separation and Detection of Seven Carbamate Pesticides in Biological Samples by Solid Phase Extraction and High Performance Liquid Chromatography

采用国产Ｃ１８柱提取生物体液中７种氨基甲酸酯类农药，用乙酸乙酯洗脱，采用二极管矩阵检测器和多通道信号检测，流动相为甲醇水（６０４０，Ｖ／Ｖ），平均回收率在６０％以上，标准偏差为１．２％～１６．３１％，变异系数为９．５９％～２３．０２％，最低检出限除尿中灭多虫和巴沙为４０～２００ｎｇ外，其余均为２０～４０ｎｇ。

Methomyl, metolcarb, propoxur, carbaryl, isoprocarb, diuron and bassa are all carbamate pesticides. In this paper, a reversed phase HPLC method is described for analysis of these carbamate pesticides in biological samples with Spherisorb C18 column(150mm×2.0mm i.d., 5μm), 60∶40 (V/V) MeOH H2O eluent and photodiode array detector. These pesticides were identified by their spectrum characteristics and retention times. Their quantity was determined by their peak area. The biological samples were extracted with C18 cartridge and eluted with ethyl acetate, and then analysed by HPLC. The sensitivity of detection for the seven carbamates was in the range of 12mg/L of blood sample and 510mg/L of urine sample. The recoveries of blood and urine samples were 70%80% and 65%82%, respectively. The method is simple, rapid and highly sensitive.