摘要
采用国产C18柱提取生物体液中7种氨基甲酸酯类农药,用乙酸乙酯洗脱,采用二极管矩阵检测器和多通道信号检测,流动相为甲醇水(6040,V/V),平均回收率在60%以上,标准偏差为1.2%~16.31%,变异系数为9.59%~23.02%,最低检出限除尿中灭多虫和巴沙为40~200ng外,其余均为20~40ng。
Methomyl, metolcarb, propoxur, carbaryl, isoprocarb, diuron and bassa are all carbamate pesticides. In this paper, a reversed phase HPLC method is described for analysis of these carbamate pesticides in biological samples with Spherisorb C18 column(150mm×2.0mm i.d., 5μm), 60∶40 (V/V) MeOH H2O eluent and photodiode array detector. These pesticides were identified by their spectrum characteristics and retention times. Their quantity was determined by their peak area. The biological samples were extracted with C18 cartridge and eluted with ethyl acetate, and then analysed by HPLC. The sensitivity of detection for the seven carbamates was in the range of 12mg/L of blood sample and 510mg/L of urine sample. The recoveries of blood and urine samples were 70%80% and 65%82%, respectively. The method is simple, rapid and highly sensitive.
出处
《色谱》
CAS
CSCD
北大核心
1997年第5期442-444,共3页
Chinese Journal of Chromatography
基金
总后"八五"科研基金
关键词
高效液相色谱
氨基甲酸酯
血
尿
固相提取
农药
high performance liquid chromatography, carbamate, blood, urine, solid phase extraction