应用气相色谱法鉴定艰难梭菌

Application of Gas Chromatography to JZ]Identification of Clostridium Difficile

应用国产气相色谱仪，选用不锈钢色谱柱，在色谱柱柱温１４０℃、检测室温度１９０℃下采用１５％改性聚乙二醇固定液（ＦＦＡＰ），以氮气作载体，将从临床肿瘤病人粪便中分离的５株艰难梭菌（Ｃｌｏｓｔｒｉｄｉｕｍｄｉｆｆｉｃｉｌｅ）菌株与１株参考株的脂肪酸代谢产物气相色谱图进行了对比分析，发现分离株与参考株的色谱图非常相似：均出现乙酸、异丁酸、丁酸、异戊酸、异己酸、丙酸、戊酸峰。其中乙酸、丁酸、异己酸峰较高；其次为异丁酸及异戊酸峰；而丙酸及戊酸峰则较低。此外，在１４ｍｉｎ时尚可见出现１个低平峰，此峰与国外Ｂｅｒｇ等报道的ｐ－甲（苯）酚峰相似。实验结果表明气相色谱法是一种简单、快速鉴定艰难梭菌的方法。

With chromatograph produced in our country, the gas chromatograms of fatty acid metabolites of five strains ofClostridium difficileisolated from stool of patients with tumor and one strain for control were compared and analysed by using stainless steel chromatographic column, 15% modified polyethylene glycol stationary phase nitrogen carriergas 140℃ column temperature and 190℃ detector temperature. The chromatograms of the isolated strains and the control strain were found to be extremely similar. All of them were peaks of acetic, propionic, isobutyric, butyric, isovaleric, valeric, and isocaproic acid. In comparison, the peaks of acetic, butyric, and isocaproic acid were higher, those of the isobutyric and isovaleric acid were medium and those of propionic and valeric acid were lower. Moreover, a low, flat peak appeared at 14th minute and was similar to that of p cresol reported by Berg et al. The results of our experiment revealed that gas chromatography may be used as a simple, quick method for the identification of Clostridium difficile.