西藏红豆杉中紫杉醇及相关紫杉烷含量的高效液相色谱分析

Reversed Phase High Performance Liquid Chromatographic (RP HPLC) Analysis of Taxol and Related Taxanes Extracted from Taxus Wallichiana Zucc

确立了紫杉醇及５种相关紫杉烷类化合物的反相高效液相色谱分析方法。最佳条件是用ＲｅｓｏｌｖｅｐａｋＣ１８柱，甲醇－水为流动相，紫外２２８ｎｍ检测。对６种化合物在反相柱上的保留机理进行了初步探讨。６种化合物的线性范围为０．１～２．０μｇ，最低检测限为０．１μｇ。

A method for determination of taxol, 10 deacetyl 7 epitaxol, cephalomannine, 10 deacetyl taxol, baccatin Ⅲ and 10 deacetylbaccatin Ⅲ, is reported and their retention mechanism is also discussed. These compounds were separated well by RP HPLC on a Resolvepak C 18 column with methanol water phosphoric acid(60∶40∶0 5) as mobile phase and UV detector at 228nm. The powdered stems were extracted with 95% ethanol , refluxed for 8 hrs and filtered and then the residue was partitioned three times between water and chloroform. The organic phase was evaporated to dryness and the residue dissolved in reagent methanol and was submitted to HPLC analysis. The results showed that there is a linear relationship between the peak area and the amount of these compounds over the range of 0 1 2 0μg. The lowest detectable concentration of these compounds was below 0.1μg.