高效液相色谱法测定非洲刺李提取物中阿魏酸二十二烷酯

Determination of Docosyl Ferulate in Extract of Pygeum Africanum Hook. by High Performance Liquid Chromatography(HPLC)

建立了非洲刺李提取物中阿魏酸二十二烷酯含量测定方法。采用ＳｐｈｅｒｉｓｏｒｂＣ１８柱，以甲醇为流动相，检测波长３２６ｎｍ，柱温４０℃，线性范围１０～１００ｍｇ／Ｌ（ｒ＝０．９９９５）。平均回收率９８．４％，ＲＳＤ为１．９８％。方法准确可靠，重现性好。

This paper reports a method for the determination of docosyl ferulate in the extract of bark of pygeum africanum Hook. by HPLC. After the sample was pretreated, the docosyl ferulate was well separated and determined on a Spherisorb C 18 column (250×4 6mm, 5μm) using a mobile phase of methanol with a flow rate of 1mL/min. The column temperature was selected at 40℃ to avoid tailing of peak. UV detection was performed at 326nm. In order to comfirm the docosyl ferulate separated from sample, the peak apex at 10.4 minute was scanned from 195nm to 360nm by photodiode array detector. Its spectrum showed the maxium absorption peak at 240nm and 326nm corresponding with the spectrum of docosyl ferulate. The linear correlation was observed from the 10mg/L to 100mg/L of docosyl ferulate (r=0.999 5). The average recovery was 98.4%±1.98%. Three batches of sample were determined.