薄层色谱溶剂系统的优化设计

Optimization of the Solvent System in Thin Layer Chromatography(TLC)

采用复合形法，根据色谱优化函数对肾上腺皮质激素类、磺胺类药物及银杏粉针剂的薄层溶剂系统进行了最优化设计。肾上腺皮质激素类药物的最佳溶剂系统为１，２－二氯乙烷－苯－甲醇（４．８１４．４２０．７８）；磺胺类药物的最佳溶剂系统为甲苯－无水乙醇－二氯乙烷－氯仿（２．３０１．４６３．３２２．９２）；银杏粉针剂的最佳溶剂系统为氯仿－甲醇－乙酸乙酯（３．３０３．２９３．４１），实验结果与计算结果一致。

Much effort in TLC has been devoted to optimization methods of solvent system in order to get best separation of components. Various optimization methods have been established, but the universality of some methods was poor. They were only applicable to samples of known components. This paper reports a complex method. When the complexity of the solvent system was determined, uniform design was used to arrange experiments, the data obtained from scanning chromatogram were input computer to get regression equation. The precision of the regression equation should be judged at first. If the precision was proper, the regression equation could be input the program of the complex method to obtain the composition of the solvent system. Optimization results were dichloroethane benzene methanol(4 81∶4 42∶0 78) for cortical hormone; toluene ethanol dichloroethane trichloromethane(2 30∶1 46∶3 32∶2 92) for sulfa drugs and trichloromethane methanol ethyl acetate(3 30∶3 29∶3 41) for ginkgo biloba. Then the verification experiment was made. The verifying test showed that the experimental results are identical with the calculated ones.