右归丸对“劳倦过度、房室不节”肾阳虚小鼠脑基因芯片的影响

Effect of Yougui Pill on Cerebral Gene Chip of Mice with Kidney-yang Asthenia Induced by Excessive Physical and Sexual Activity

目的研究右归丸对"劳倦过度、房室不节"肾阳虚小鼠模型脑基因的影响,以期从基因水平上研究其药物作用机理。方法对照组、模型组和治疗组雄性小鼠各为10只、15只和10只。对照组和模型组每日每只灌胃蒸馏水0.5ml,治疗组每日每只予右归丸混悬液0.5ml,模型组和治疗组雄雌鼠比例1∶6同笼喂养且雄鼠每日游泳30～40分钟共4周,以诱导产生"劳倦过度、房室不节"肾阳虚证。用36K鼠脑芯片检测3组鼠脑基因,并各以二者荧光信号相对强度比值≥2和≤0.5筛选差异显著基因。结果模型组/对照组共筛选出186个差异表达基因,其中上调基因150个,下调基因36个。治疗组/模型组筛选出155个差异表达基因,其中上调基因64个,下调基因91个。模型组/对照组上调而治疗组/模型组下调的基因有32个,主要是炎症/免疫、神经传递/信号转导等基因。模型组/对照组下调而治疗组/模型组上调的基因有12个,主要是与黑色素、孤儿核受体相关的基因。结论右归丸可使肾阳虚小鼠模型显著下调的黑色素显著上调,改善与孤儿核受体有关激素的作用并促进细胞增殖。

Objective To study the functional mechanism of Yougui Pill （Right Restoring Pill） through its effect on cerebral gene of mice models with kidney-yang asthenia caused by excessive physical and sexual activity. Methods There were 10, 15, 10 male mice in the control, model and treatment groups respectively. The control and model groups were treated with 0.5ml permouse perday distilled water by gavage while the treatment group was given 0.5ml permouse perday suspension of Yougui Pill. In the model and treatment groups, the ratio of male and female was 1 ： 6, feeding in the same cage, and the males were swimming everyday for 30～40 minutes, 4 weeks in succession, to induce the kidney-yang asthenia. The 36K mouse cerebral gene chips were used to measure the brain genes of mice of 3 groups. The intensity ratio of fluorescence signals ≥2 and ≤0.5 were used as the scale to screen out the remarkably different genes. Results In the model and control groups, totally 186 gene with different expressions were screened out, including the 150 up-regulated genes and 36 down-regulated genes. In the treatment and model groups, 155 gene with different expressions were screened out, including the up-regulated 64 genes and 91 down-regulated genes. There were 32 genes up-regulated in the model and control groups but down regulated in the treatment and model groups, mainly involving the inflammation perimmunity and neurotransmissions persignal transduction genes. There were 12 genes down-regulated in the model and control groups but up-regulated in the treatment and model group, mainly involving the hormone related genes of melanin and orphan nuclear receptor. Conclusion Yougui Pill may make the notably down-regulated genes of melanin markedly up-regulated in mice models of kidney-yang asthenia, improving the effect of hormone related orphan nuclear receptor and promoting the cellular proliferation.