比生长速率和氮源对毕赤酵母生产重组鲈鱼生长激素的影响

Effect of Specific Growth Rate and Nitrogen Source on the Production of Recombinant Lateolabrax japonicus Growth Hormone by Pichia pastoris

对毕赤酵母胞内表达重组鲈鱼生长激素(rljGH)的发酵罐上生产进行了研究。建立了指数流加甲醇的策略并考察了不同比生长速率对rljGH生产的影响。结果表明,随着比生长速率的增加,平均比生产速率相应增加,但是胞内持续积累rljGH的时间减少。最大比rljGH产量(0.58mg/gWCW)在比生长速率为0.029/h时获得。进一步考察了在诱导阶段添加硫酸铵、蛋白胨和酵母抽提物的影响。结果表明,添加硫酸铵和蛋白胨对于rljGH生产没有显著影响;添加2.5g/L酵母抽提物有助于胞内rljGH的积累,并使胞内积累持续时间由17h增加到23h,提高了发酵稳定性。

The bioreactor production of recombinant Lateolabrax japonicus growth hormone （rljGH） expressed intracellularly by Pichia pastoris was investigated. A strategy of feeding methanol at the exponential rate was established and the effect of specific growth rate on the rljGH production was examined. The results indicated that the average specific production rate increased and the rljGH production duration decreased as the specific growth rate increased. The maximum specific rljGH production （0.58 mg/g WCW） was achieved at a specific growth rate of 0.029/h. The effect of supplementing ammonium sulfate, peptone and yeast extract on the rljGH production was further investigated. The results indicated that the effects of ammonium sulfate and peptone were not significant. Supplementing yeast extract of 2.5 g/L was advantageous for the rljGH production. The duration of the rljGH production was increased to 23 h from 17 h and the fermentation stability of run-to-run could be improved.