摘要
采用刺囊毛霉AS3.3450对甘草次酸进行微生物转化,生成的主产物经分析鉴定是7β-羟基甘草次酸。采用高效液相色谱方法,以C18-ODS为色谱柱,甲醇-0.03%三氟乙酸水溶液(梯度洗脱)为流动相,检测波长为:254nm,测得在160r/min、27°C的转化条件下,底物加量为130mg/L,转化时间为9d时,主产物得率最高为712mg/g甘草次酸,且在选定色谱条件下7β-羟基甘草次酸的线性范围良好,平均加样回收率为98.1%,平均标准偏差(RSD)为2.37%。
The major product of microbial transformation of glycyrrhetinic acid by Mucor spinosus AS3.3450 has been identified as 7β-hydroxyglycyrrhetinic acid on the basis of its spectral data. 7β-hydroxyglycyrrhetinic acid was directly determined by HPLC. Methanol and ultra pure water containing 0.03% trifluoroacetic acid was used as the mobile phase on C18 column, the UV detection wavelength was 254 nm. The maximum transformation rate observed was 712 mg 7β-hydroxyglycyrrhetinic acid/g glycyrrhetinic acid on day 9 under 27℃, 160 r/min and substrate at a concentration of 130 mg/L. Results show that the content of 7β-hydroxyglycyrrhetinic acid indicates a good linearity under these conditions with a average recovery of 98.1% and a RSD of 2.37%.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第10期1664-1667,共4页
Microbiology China
基金
北京市属市管高等学校人才强教计划
