摘要
利用实验室自行从亚洲传统发酵食品虾酱中筛选到一株产纤溶酶能力较强的芽孢杆菌(Bacillus sp.nov.SK006)进行发酵,考察培养基组成及培养条件对该菌种产纤溶酶能力的影响,结果表明该菌株产酶最佳的组分(质量浓度)为:葡萄糖20g/L,胰蛋白胨30g/L,Na2HPO4·12H2O12g/L,NaH2PO4·2H2O1.3g/L,MgSO4·7H2O1.0g/L;培养条件:种龄18h,接种量3%,发酵周期24h,初始pH为7.0,摇床转速180r/min,发酵温度37℃,在此条件下发酵液纤溶活性(以Plasmin为标准)高达2.63U/mL,优化后纤维蛋白溶解酶的产量是优化前0.70U/mL的3.76倍。
The medium composition and fermentation conditions for fibrinolytic enzyme production were optimized in the research. The maximal yield of fibrinolytic enzyme（2.63 U/mL） could be obtained in the medium with concentrations of glucose, tryptone, Na2HPO4·12H2O, NaH2PO4·2H2O and MgSO4.7H2O set at 20, 30, 12, 1.3 g/L and 1.0 g/L, respectively, under the optimized cultivation conditions of pH 7.0, rotation speed 180 r/min and inoculation size 3% in 50 mL of culture medium in 250 mL shake flasks at 37℃ for 24 h. The fibrinolytic enzyme production of Bacillus sp. nov. SK006 was 3.76 times of the control.
出处
《食品科技》
CAS
北大核心
2008年第10期1-5,共5页
Food Science and Technology
基金
江苏省国际技术合作计划项目(BZ2007036)
关键词
复合酶
鱿鱼
蛋白质
水解产物
fibrinolytic enzyme
Bacillus sp.
liquid fermentation
optimum condition
