摘要
以鸡蛋清为原料,通过阳离子交换树脂法提取溶菌酶,确定搅拌吸附条件,蛋清树脂比5:1、pH7.0、室温下慢速搅拌吸附4h、用1.0mol/L NaCl洗脱,收集洗脱液,超滤、浓缩,冷冻干燥得到溶菌酶成品。最终酶收率为89.78%、酶活力为14817U/mg。溶菌酶配制成0.05%溶液喷涂在出炉冷却10min的蛋糕表面,可延长产品的贮存期。
To get lysozyme from albumen by ion exchange resin adsorptive process, process and condition as follows: rate of albumen and resin is 5:1, pH7.0, slowly stir and adsorb 4 hours at room temperature, elute with 1.0 mol/L NaCl, collect the eluant, ultrafiltrate, concentrate, cryodesiccate then get antalzyme product. Last yield of lysozyrne is 89.78%, enzyme activity is 14817 U/mg. 0.05% solution of lysozyme was spray painting to the surface of cake which cooling 10 minutes after bake, the keeping time of cake was prolong.
出处
《食品科技》
CAS
北大核心
2008年第10期167-169,共3页
Food Science and Technology
关键词
溶菌酶
鸡蛋清
树脂吸附法
防腐
焙烤食品
lysozyme
albumen
ion exchange resin adsorptive process
antisepticise
baking food