阿斯美胶囊中四种组分的高效液相色谱分析

HPLC Determination of the Four Active Ingredients in Asmeton Strong Capsules

建立了反相高效液相色谱法测定阿斯美胶囊中氨茶碱、盐酸甲氧非那明、马来酸氯苯那敏及那可汀四种组分的含量。色谱柱为ＳｐｈｅｒｉｓｏｒｂＣ８，５μｍ，２００ｃｍ×４０ｍｍＩＤ，流动相为体积分数０５％的三乙胺溶液（磷酸调节ｐＨ５５）＋乙腈＋甲醇（５５０＋３６８＋８２），检测波长为２６４ｎｍ，线性范围分别为：氨茶碱３１２５～２５０ｍｇ／Ｌ，γ＝０９９９６，盐酸甲氧非那明１５６２５～１２５０ｍｇ／Ｌ，γ＝１００００，马来酸氯苯那敏２５～２００ｍｇ／Ｌ，γ＝０９９９９，那可汀８７５～７００ｍｇ／Ｌ，γ＝０９９９９；回收率（ｎ＝３）分别为１０１２％，１００８％，９９９％，９９５％；精密度：日内平均ＲＳＤ（ｎ＝６）分别为０７％，０４％，０４％，０６％；日间平均ＲＳＤ（ｎ＝３）分别为１０％，１０％，１１％，１２％。

The active ingredients in Asmeton strong capsules are aminophylline,methoxyphenamine HCl,chlorpheniramine maleate and noscapine They were determined simultaneously by a reversed phase HPLC system by using a 20 cm×4 0 mm I D column packed with Spherisorb C8,5 μm,a mixture mobile phase consisting of 0 5%(φ B)triethylamine solution(adjusted with phosphoric acid to a pH of 5 5)acetonitrile methanol(550∶368∶82)and a UV detector at 264 nm Some parameters of the method are listed below: Limit of linearity mg/LRecovery %RSD %within daybetween days[WT5”BZ〗Aminophylline31 25～250101 20 71 0γ=0 9996Methoxyphenamine HCl156 25～1250100 80 41 0γ=1 0000Chlorpheniramine maleate25～20099 90 41 1γ=0 9999Noscapine87 5～70099 50 41 2γ=0 9999