急性白血病患者JAK家族基因的表达及临床意义

Clinical Significance of the Expression of JAKs Genes in Cells of Acute Leukemia Patients

目的探讨不同时期急性白血病(AL)细胞中JAKs基因的表达情况及其临床意义。方法将入选患者分为初治组、复发组、完全缓解组(CR组)和正常对照组(NC组),RT-PCR方法检测AL患者和正常对照者骨髓MNC中JAK1～3和TYK2 mRNA的表达。结果(1)AL患者JAK2、JAK3、TYK2 mRNA平均表达水平较CR组和NC组差异有统计学意义(P<0.05)。初治AL患者JAK1 mRNA表达水平较NC组略增高,但差异无统计学(P>0.05),复发AL患者JAK1 mRNA表达水平较NC组差异有统计学意义(P<0.05);(2)TYK2阳性组的AL患者缓解率较TYK2阴性患者差异有统计学意义(P<0.05)。结论JAK家族基因在白血病患者的高表达可能在白血病的发生发展中起重要作用。急性髓性白血病(AML)和急性淋巴细胞白血病(ALL)在激活JAK/STAT信号传导通路的方式有所不同。

Objective To investigate the expressions of JAKs gene in cells of patients with acute leukemia （AL） in different stages and their clinical significance. Methods The patients enrolled were divided as initial treatment group, relapse group and complete remission （CR） group, with a normal control （NC） group. RT - PCR was used in assay of expressions of JAK1 - 3 and TYK2 mRNA of bone marrow mononuclear ceils （ BM - MNCs）. Results The average expression levels of JAK2, JAK3 and TYK2 mRNA in the initial treatment and relapse groups were significantly higher than those in the CR and NC groups （ P 〈0. 05）. The expression level of JAK1 mRNA was slightly higher in the initial group than in the NC group, but with no significant difference （ P 〉0.05）. The expression level of JAK1 mRNA was higher in the relapse group than in the NC group, with a significant difference （ P 〈 0. 05）. There was significant difference in remission rate of AL between the patients with positive TYK2 expression and with negative TYK2 expression （ P 〈 0. 05）. Conclusion The high expression of JAKs may play an important role in development of leukemia. Acute myeloid leukemia and acute lamphoblastic leukemia have different modes of JAK/ STAT signaling pathway.