人肺腺癌发生过程中的细胞核大小及其分布

Nuclear size and its distribution in human lung adenocarcinogenesis

用计算机图像分析仪对尸检正常支气管粘膜上皮细胞、支气管纤维镜活检的支气管粘膜异型性或不典型性增生上皮细胞（即癌前病变）及高、低分化肺腺癌组织的细胞核进行体积权重平均体积、截面面积及其分布的分析。结果：细胞核体积权重平均体积及截面面积肺腺癌组大于癌前病变组和正常组，癌前病变组大于正常组（Ｐ＜０．０５）。细胞核体积权重平均体积的分布显示：高、低分化肺腺癌的细胞核在≤２０８μｍ３组段中占百分率明显低于正常组及癌前病变组，而细胞核＞５０５μｍ３组段中则明显高于正常组，且低分化肺腺癌组同时高于癌前变组，以上变化均具有显著差异（Ｐ＜０．０５）；并且细胞核大小随癌变发展而呈现逐渐增大的趋势。结论：对正常组织与癌前病变及高、低分化肺腺癌细胞核大小及其分布定量分析所得出的量化指标，可望供肺腺癌早期诊断和鉴别诊断提供参考。

A computerized image analysing system was used to study quantitatively the nuclear volume and area of cells which were sampled from 15 cases of normal bronchial epithelium from autopsy,8 cases of atypical or dyoplasia proliferated bronchial epithelium(precancer)and 8 cases of poorly and well differentiations lung adenocarcinoma tissues from biopsy,respectively.The results showed that the nuclear volumes and areas of the lung adenocarcinoma cells were larger than that of the precarcinoma and normal cell (P<0.05).The nuclear volume and area of the precarcinoma cell were also larger than that of the normal(P<0.05).There was no statistical significance in the nuclear volume and areas of the cancer cells between the well and poorly differentiated human lung adenocarcinoma(P>0.05).In the well and the poorly differentiated lung adenocarcinoma,the percentages of small nuclei(≤208μm3)were lower,but the percentages of the large nuclei (>505μm3)were singnificantly higher than that of the precancer and normal groups.Our findings indicated that quantitative analysis of nuclear morphometry would provide good evidence for the early diagnosis of lung cancer in different stages.