真菌寡聚糖对大豆生长及植保素产生的影响

Effect of Fungal Oligosaccharides on Growth and Phytoalexin Inducement of Soybean

利用热解法从DL1805真菌壁上提取寡聚糖(DLO),用浸种法处理大豆种子,处理时间为4 h,自然条件下播种于盛有灭菌蛭石的塑料筐内,观察DLO对大豆生长的影响。结果显示:用浓度为1、5、50 mg.L-1的寡聚糖处理大豆种子,生长9 d后,处理植株生长普遍好于对照植株,并且随处理浓度的增大诱导生长的效果越好,浓度为50 mg.L-1的效果最为显著,与对照相比,茎粗增加5%以上,株高增加17%以上,鲜重增加15%以上,干重增加5%以上,茎干重增加2.8%以上。DLO能诱导大豆植保素的产生,在5个处理浓度中随浓度的加大诱导效果也逐渐增强,100 mg.L-1的时候OD(A286)达到1.103。因此,DL1805菌壁降解寡聚糖能较好地诱导大豆的生长,并且还能诱导植保素的产生提高大豆的抗病能力。

Oligosaccharide from Dalian fungi （DLO） was obtained through thermal cracking of fungal cell wall. In order to study the effects of DLO on growth traits and phytoalexin inducement of soybean,the concentration of 1,5,50 mg·L^-1 DLO were used to treat soybean for 4 h with seed-immersion method. The results indicated that the plants treated with DLO grew better than that of control. The efficacy of DLO increased with concentration. Under 50 mg·L^-1, the stems were 5% thicker, 17% higher and 2.8% heavier than that of control nine days after treatment. The inducement of DLO also increased with concentration and the OD value of 100 mg·L^-1 reached 1. 103. The results indicate that DLO has the effeet of promoting growth and inducing phytoalexin production of soybean.