营养水平对DLY杂交猪肉质性状及H-FABP基因表达的影响

Effects of Nutrition Levels on Heart Fatty Acid-binding Protein Gene Expression and Fatty Metabolism in Muscle of Pigs

将24头起始重为(64.24±2.66)kg的健康DLY杂交猪随机分为2个处理,分别饲喂高、低营养水平的日粮,研究高、低营养水平对育肥猪肉质性状及H-FABP基因表达的影响。结果表明:与低营养水平相比,高营养水平提高了日增重(P=0.273)和饲料报酬(P=0.001);降低营养水平可提高H-FABP基因表达量(P=0.288)和IMF含量(P=0.09),增加肉的嫩度(P=0.026);降低营养水平,还提高了肌肉组织中苹果酸脱氢酶和脂蛋白脂酶的活性(P>0.05)。因此,营养水平可以影响肌肉组织脂肪代谢和H-FABP基因的表达。

Intramuscular fat content is generally associated with sensory quality and acceptability of fresh pork. This study was conducted with the RT-PCR method to determine effects of nutrition levels （nutrient density） on heart fatty acid-binding protein（H-FA BP） gene expression and fatty metabolism in muscle in DLY （Duroc xLandrance xYorkshire） pigs. A total of 24 DLY pigs with BW （64.24±2.66） kg were fed two diets of either high （DE= 13.81MJ/kg, CP= 14.19%） or low （DE=12.55 MJ/kg, CP= 11.08%）nutrition level. Six pigs were slaughtered in each group when pigs weighed 100 kg. The results indicated that the ADG was increased （P 〉 0.05）and F/G was reduced （P 〈 0.01） for pigs fed high nutrient level. H-FABP gene expression and intramuscular fat content in skeletal muscle were increased by 26.18% （P = 0.288 ） and 37.28%（P = 0.09）, and meat tenderness was increased by 15.05%（P = 0.026）and fatty metabolic enzymes MDH and LPL in muscle were increased by 11.90%（P= 0.288 ）and 11.77%, and FFA in blood plasm was reduced （P 〉 0.05 ） for pigs fed diet of low nutrition level compared to pigs fed diet of high level. It is concluded that H-FABP gene expression and fatty metabolism in muscle are affected by dietary nutritional level.