摘要
将24头起始重为(64.24±2.66)kg的健康DLY杂交猪随机分为2个处理,分别饲喂高、低营养水平的日粮,研究高、低营养水平对育肥猪肉质性状及H-FABP基因表达的影响。结果表明:与低营养水平相比,高营养水平提高了日增重(P=0.273)和饲料报酬(P=0.001);降低营养水平可提高H-FABP基因表达量(P=0.288)和IMF含量(P=0.09),增加肉的嫩度(P=0.026);降低营养水平,还提高了肌肉组织中苹果酸脱氢酶和脂蛋白脂酶的活性(P>0.05)。因此,营养水平可以影响肌肉组织脂肪代谢和H-FABP基因的表达。
Intramuscular fat content is generally associated with sensory quality and acceptability of fresh pork. This study was conducted with the RT-PCR method to determine effects of nutrition levels (nutrient density) on heart fatty acid-binding protein(H-FA BP) gene expression and fatty metabolism in muscle in DLY (Duroc xLandrance xYorkshire) pigs. A total of 24 DLY pigs with BW (64.24±2.66) kg were fed two diets of either high (DE= 13.81MJ/kg, CP= 14.19%) or low (DE=12.55 MJ/kg, CP= 11.08%)nutrition level. Six pigs were slaughtered in each group when pigs weighed 100 kg. The results indicated that the ADG was increased (P 〉 0.05)and F/G was reduced (P 〈 0.01) for pigs fed high nutrient level. H-FABP gene expression and intramuscular fat content in skeletal muscle were increased by 26.18% (P = 0.288 ) and 37.28%(P = 0.09), and meat tenderness was increased by 15.05%(P = 0.026)and fatty metabolic enzymes MDH and LPL in muscle were increased by 11.90%(P= 0.288 )and 11.77%, and FFA in blood plasm was reduced (P 〉 0.05 ) for pigs fed diet of low nutrition level compared to pigs fed diet of high level. It is concluded that H-FABP gene expression and fatty metabolism in muscle are affected by dietary nutritional level.
出处
《中国畜牧杂志》
CAS
北大核心
2008年第19期26-29,共4页
Chinese Journal of Animal Science
基金
国家973项目资助(2004CB117500)
关键词
H—FABP
基因表达
肉质
营养水平
脂质代谢
猪
H-FABP
gene expression
pork quality
nutrition level
fatty enzymatic activity
pig