泰国葛组织培养和快速繁殖体系优化研究

Research on optimization system on tissue culture and rapid propagation of Pueraria mirifica

目的:研究泰国葛的组织培养与快速繁殖技术。方法:以嫩枝为外植体,通过调整基本培养基、生长调节剂种类和配比、以及向培养基中添加特殊化合物,以期选择出适合的诱导、继代增殖和生根培养基配方。结果:MS+IBA 0.05 mg.L-1+BA 0.5 mg.L-1利于诱导出芽,可用于初代培养;MS+IBA 0.02 mg.L-1+BA 0.2mg.L-1和MS+BA 0.1 mg.L-1可用于增殖和继代培养,25 d繁殖系数3.0;1/2 MS+IBA 0.1 mg.L-1+IAA 0.2mg.L-1+C(特殊化合物)10 mg.L-1适于诱导生根获得再生植株,生根率76.9%。生根苗春、夏季移栽,成活率81.0%。结论:本方法可用于泰国葛种苗的快速繁殖。

Objective： To study the tissue culture and rapid-proliferation techniques of Pueraria mirifica. Method： The tender branch were used as explants and cultivated in different media. The optimum media for inducing buds, proliferation and rooting were selected by adjusting the kinds and doses of plant hormones and special compounds. Result： The medium of MS ＋ IBA 0.05mg·L^-1 ＋ BA 0.5 mg·L^-1 was suitable for buds inducing and could be used in the first generation cultivation; MS ＋ IBA 0.02 mg·L^-1 ＋ BA 0. 2mg·L^-1 and MS ＋ BA0. 1mg·L^-1 were employed by turns in subculture, 25 days propagation coefficient was 3.0; and the medium of 1/2MS ＋ IBA 0.1 mg·L^-1 ＋ IAA 0.2mg·L^-1＋ C （ special compound） 10 mg·L^-1 was used for roots inducing, the rooting rate was 76.9%. Rooting plantlets were transplanted in spring and summer; the surviving rate was 81.0%. Conclusion： This technique system could be employed for rapid propagation of P. mirifica.