毛建草化学成分的研究

Studies on chemical constituents of Ranunculus japonicus

目的：对六盘山地区毛茛科植物毛建草Ranunculus japonicus的化学成分进行分离和鉴定。方法：采用超声波乙醇浸提法提取后分别以石油醚、醋酸乙酯、正丁醇萃取，石油醚和醋酸乙酯部位用硅胶柱色谱及Sepha-dexLH-20进行分离纯化，通过理化性质和波谱技术鉴定结构。结果：从毛建草中分离鉴定了10个化合物，分别鉴定为滨蒿内酯（scoparone，1）、小麦黄素（tricin，2）、原儿茶酸（protocatechuicacid，3）、木犀草素（1uteolin，4）、白头翁素（anemonin，5）、东莨菪内酯（scopoletin，6）、5-羟基-6，7-二甲氧基黄酮（5-hydroxy-6，7-dimethoxyflavone，7）、小毛茛内酯（tematolide，8）、5-羟基-7，8-二甲氧基黄酮（5-hydmxy-7，8-dimethoxyflavone，9）。结论：化合物1-9均为首次从该植物中分离得到。

Objective： To isolate and determine the chemical constituents of Ranunculus japonicus in Liupan Mountain, Ningxia province, China. Method： The herb was extracted with ethanol by ultrasonic bath. The extractives were divided to petroleum ether, ethyl acetate, n-butanol parts. The first two parts were separated and purified with silica gel and Sephadex LH -20 column chromatography. The structures of the separated compounds were idnetefied by physical and chemical properties and spectral analysis. Result： Nine compounds were isolated and identified as follows： scoparone （1）, tricin （2）, protocatechuic acid（3）, luteolin （4）, anemonin （5）, scopoletin （ 6 ）, 5-hydroxy-6, 7-dimethoxyflavone （ 7 ）, ternatolide （ 8 ）, 5-hydroxy-7, 8-dimethoxy-flavone （ 9 ）. Conclusion： Compounds 1-9 were isolated from Ranunculus japonicus for the first time.