左金丸抗肿瘤作用及对S180荷瘤小鼠血清肿瘤标志物的影响

Anti-cancer activity of Zuojinwan in vivo and influence to tumor markers in mice transplanted with sarcoma 180

目的:研究左金丸的体内抗肿瘤作用,并探讨其对S180荷瘤小鼠血清中肿瘤标志物酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、肌酸激酶(CK)、醛缩酶(ALD)和乳酸脱氢酶(LDH)活力的影响,并与其配伍方药黄连和吴茱萸进行比较。方法:建立S180实体瘤模型,昆明种小鼠右腋下接种0.2 mL S180细胞悬液(5×106个/mL细胞)后,随机分成5组,24 h后分别给予左金丸(850.8 mg.kg-1)、黄连(729.2 mg.kg-1)和吴茱萸(121.6 mg.kg-1)提取物10 d,观察小鼠体重、脾指数,计算抑瘤率和生命延长率(ILS),并检测不同组给药小鼠血清中ACP,AKP,CK,ALD和LDH的活力。结果:左金丸对移植性S180肿瘤的抑瘤率为50.54%,对小鼠的ILS为64.91%。同时左金丸可显著提高小鼠血清ACP(126.72±11.16)U.100 mL-1和AKP(67.27±13.49)U.100 mL-1的活力,且显著降低小鼠血清CK(20.65±4.28)U.mL-1,ALD(319.13±53.87)U.L-1和LDH(1029.04±468.56)U.L-1的活力,与黄连和吴茱萸单独给药有着显著性差异(P<0.01)。结论:左金丸方中黄连和吴茱萸能产生明显的配伍协同抗肿瘤作用,对血清5种肿瘤标志物的影响可能是其抗肿瘤作用的潜在机制。

Objective： To study the anticancer action of Zuojinwan in mice transplanted with sarcoma 180 in vivo, and detect the activities of five kinds of tumor markers （TM） including acid phosphotase （ACP）, alkaline phosphotase （AKP）, creatine kinase （CK）, aldolase （ALD） and lactate dehydrogenase （LDH） in serum compared with Coptis chinensis and Evodia tutaecarpa. Method： The transplanted S180 tumor mice model was established, and the mice were divided randomly five groups. The extract of Zuojinwan （850. 8 mg· kg^-1 ）, C. chinensis （729. 2mg· kg^-1 ） and E. rutaecarpa （ 121.6 mg· kg^-1 ） were administrated, respectively for 10 d. Then, the changes of body weight, spleen index of mice, the inhibition rates of tumor, and the increase of life span （ILS） were all tested. In addition, the activities of ACP, AKP, CK, ALD and LDH on different test groups were also determined. Result： Zuojinwan could inhibit the S180 tumor growth significantly with the inhibition rate of 50.54% and the ILS of mice reached to 64. 91%. Meanwhile, the activities of ACP （126. 72 ± 11.16） U· 100 mL^-1 and AKP （67. 27 ± 13.49） U · 100 mL^-1 were increased, and the activities of CK （20. 65 ±4. 28） U·mL^-1, ALD （319. 13 ±53. 87）U·L^-1 and LDH （ 1 029. 04 ±468. 56） U·L^-1 were decreased significantly by Zuojinwan treated group compared with C. chinensis and E. rutaecarpa treated groups （P 〈0. 01 ）. Conclusion： In the prescription of Zuojinwan, the enhancement of compatibility of anticaneer activity was observed by the interaction of C. chinensis and E. rutaecarpa. The mechanism might be in according with to influence the activities of the five kinds of tumor markers （TM） in mice serum.