抗肿瘤药物与TRAIL联用对喉癌HEP-2细胞株增殖、凋亡的影响

Effects of trall combined with 5-Fu and DDP on proliferation and apoptosis of HEP-2 cell line

目的探讨化疗药物顺铂(DDP)、5氟尿嘧啶(5-Fu)单独及与TRAIL联合对HEP-2细胞株增殖、凋亡的影响。方法MTT法检测其增殖抑制作用,FCM法检测细胞凋亡率。结果TRAIL与5-Fu、DDP联合作用于HEP-2细胞株时,其抑制率、凋亡率高于单独作用时的抑制率和凋亡率。结论TRAIL与5-Fu、DDP联合作用有协同抑制HEP-2细胞株增殖、诱导HEP-2细胞株凋亡的作用。

Objective To observe the effects of different concentrations of TRAIL and chemotherapeutic drugs（5-Fu, DDP）on HEP-2 cell lines proliferation,apoptosis,both individually and jointly. Methods MTT was used to detect the inhibition rate, FCM was used to detect the apoptosis rate of cell. Results The inhibition rate and apoptosis rate of combination was statistically significantly increased,comparing with individually. Conclusion The inhibition of proliferation and apoptosis of HEP 2 cell line can be induced by TRAIL,and the effects were higher by TRAIL combined with 5 Fu and DDP.