前列腺素E_1对肿瘤坏死因子水平及磷脂酶A_2活性的影响

Effects of prostaglandin E1 on the serum tumor necrosis factor level and phospholipase A2 activity

为探讨前列腺素E1（PGE1）保护肝细胞的作用机制，本实验应用D-氨基半乳糖及内毒素复制急性肝衰竭（AHF）模型，观察PGE1对血清肿瘤坏死因子（TNF）水平及肝组织匀浆磷脂酶A2（PLA2）活性的影响，结果显示：PGE1治疗组鼠血清TNF含量及肝组织匀浆PLA2活性明显低于对照组（P＜0．01），肝组织坏死面积也小于对照组（P＜0．01），提示PGE1保护肝细胞作用与降低TNF合成及抑制PLA2活性有关。

To investigate the mechanism in which prostaglandin E1 (PGE1 ) protects hepatic cell from damage,rat was injected intraperitoneally with D-Galactosamine (1100mg/kg) plus endotoxin (20μg/each rat) to induce acute hepatic failure.The effects of PGE1 on serum tumor necrosis factor (TNF) level and Phospholipase A2 (PLA2 ) activity in the liver homogenate was observed. As a result, the TNF level and PLA2 activity decreased significantly in the group received PGE1 (P < 0. 01 ). Liver necrosis area was obviously smaller than that of the control group (P< 0. 01). In conclusion, these results suggested that PGE1 Protect hepatic cell ftom injury through inhibiting TNF production and suppressing PLA2 activity.