大鼠肾组织血小板衍生的生长因子与肾炎发病的关系

The relationship between platelet-derived growth factor within rat kidneyand the development of nephritis

用免疫细胞化学方法研究了大鼠肾毒血清肾炎（nephrotoxicnephritis,NTN）发病头7天肾组织中血小板衍生的生长因子（platelet－derivedgrowthfactor，PDGF）增生细胞核抗原（proliferatingcellmuclearantigen,PCNA），和单核／巨噬细胞的变化以及检测这些细胞之间及其与肾功能之间的相关性。结果表明，NTN大鼠发病第1天，肾小球内未能检出PDGF.发病第3天，肾小球内出现PDGF，分布在血管间质区，与浸润的单核／巨噬细胞的分布一致，两者细胞数呈明显正相关。发病第7天，PDGF染色阳性细胞数与PCNA，蛋白尿和血肌酐亦呈显著正相关。提示NTN时，PDGF并不参与肾小球最初的免疫损伤机制，而可能为损伤后浸润的单核细胞和受刺激的血管间质细胞所释放，并在肾炎发展过程中起作用。

We have studied the distribution of immunoreactive PDGF platelet-derived growth factor,PCNA(proliferating cell nuclear antigen) and Ia common determinant OX-6 within rat rat kidneys and theircorrelation at initial stage of an accelerated form of nephrotoxic serum nephritis (NTN). On the first day of NTN,no PDGF was observed in the glomeruli. On the third day, PDGF was in a glomerular mesangial distributionsimilar to that of infiltrating monocytes/macrophages.The PDGF positive cells were closely cormlated withmonocytes/ macrophages. On the seventh day PDGF were correlated with monocytes macrophages PCNA, proteinuria and serum creatinine. These results suggested that PDGF didn' t play any role in the initial immuneinsult of glomerulus in NTN, but initial immune-insult may stimulate monocytes/macrophages and mesangialcells to crease PDGF and theatrer PDGF play an important ladle in the profusion of NTN.