益肾达络饮对实验性自身免疫性脑脊髓炎β淀粉样前体蛋白的影响

Effect of Yishendaluo Decoction on APP in the Mice of Experimental Autoimmune Encephalomyelitis

目的:研究中药复方益肾达络饮对PLP139-151诱导的实验性自身免疫性脑脊髓炎小鼠中枢神经系统内轴突病变标记物β淀粉样前体蛋白(β-amyloid precursor protein,APP)的影响,探讨益肾达络饮在神经系统炎性脱髓鞘疾病中的作用机制。方法:采用免疫组化技术,测定各组小鼠中枢神经组织中APP蛋白含量。结果:APP蛋白表达正常组与模型组、激素组、中药组比较均有极显著性差异(P值均<0.01),模型组与中药组比较有极显著性差异(P值均<0.01),与激素组比较有显著性差异(P<0.05),激素组与中药组比较有显著性差异(P<0.05)。结论:EAE小鼠APP表达升高,提示存在轴索损害。醋酸泼尼松、益肾达络饮均降低EAE小鼠中枢神经组织内APP的表达,且益肾达络饮优于醋酸泼尼松。益肾达络饮自身免疫性脑脊髓炎小鼠保护作用可能与下调APP、抑制轴突变性有一定关系。

Objective： To investigate the effect of Yishendaluo decoction on β-amyloid precursor protein （APP） in central nervous system of the mice with PLP139-151-induced experimental autoimmune encephalomyelitis and to explore the mechanism of Yishendaluo decoction on the inflammatory demyelinating diseases in the nervous system. Methods： Female mice were evenly randomized into normal, model, control and treatment groups. The contents of APP protein in the four groups above were detected with method. Results： The contents of APP in model, control and treatment groups were all significantly higher than those in normal group （ P 〈 0.01, 0.01 and 0.01 respectively）. Compared with model group, the augmentation contents of APP in central nervous system were reduced significantly in both control and treatment groups （P 〈0.05 and P 〈0.01 differences between the two groups above （ P 〈 0.05）. respectively）, and it was shown statistically significant Conclusions ： The results of present study indicate that Yishendaluo decoction can protect the mouse from the experimental autoimmune encephalomyelitis by attenuating the content of APP and inhibiting axonal degeneration.