毛葡萄组织培养的脱毒技术

Techniques of De-Virus of Vitis quinquangularis Rehd on Tissue Culture

以“九龙二号”毛葡萄幼芽为外植体，诱导无根试管苗，研究其脱病毒和植株再生技术。结果表明，采用白天37℃处理8h（光照强度40μmol·m^-2·s^-1），晚上22℃处理16h的变温方法处理10-15d，取0．8～1．0mm茎尖接种于B5＋BA 0．5mg／L＋IAA 0．1mg／L培养基中，茎尖存活率可达80％以上，且脱毒效果达100％。1／2 MS＋BA 0．2mg／L＋IAA 0．6mg／L诱导根的效果最佳，生根率达86．7％。

Taking the major cultivars named Jiulong No. 2 as the explant induce rootless plantlet in order to study techniques of viruses elimination and plant regeneration. Theresults showed that the survival rate could reach to 80% and the de-virus rate could achieve to 100% ,if the cultivars were cultivated with poikilothermic treatment the stem apex （length：0. 8- 1.0 mm） of cultivars should be cultivated in culture medium （B5＋BA 0. 5 mg/L＋IAA 0. 1 mg/L）,being kept for 10-15 d at 37 ℃ for 8 h （strength of illumination：40 μmol·m^-2·s^-1） in the daytime,and 22 ℃ for 16 h at night. The optimum effect of buds inducing was 1/2 MS＋BA 0. 2 mg/L ＋IAA 0. 6 mg/L,which could make the rate of rooting reach 86.7%.